Peristaltic Liquid Pump
Flow range:0.0053-6000ml/min
2.Basic peristaltic pump:LabM series
Flow range:0.0053-3100ml/min
3.Industrial peristaltic pump
Speed range:0.1-600rpm
Description
Technical Parameters
Peristaltic liquid pump, usually referred to as peristaltic pump, also known as constant flow pump and hose pump, is a unique working principle of liquid transport device. Peristaltic pump realizes the transfer of liquid through peristaltic movement, which is mainly composed of a soft pipe and a roller or rotor that can squeeze the pipe. When working, the roller or rotor will roll along the length direction of the pipeline and squeeze the pipeline, making the section of the pipeline smaller and closed. The liquid will be subjected to extrusion pressure, which will cause the liquid inside the pipeline to form a peristaltic wave. The peristaltic wave will move from the feed port to the discharge port, and push the liquid to the discharge port. The liquid is pumped into the pipe and then transported along the pipe to the discharge port as the peristaltic wave advances.
Storage environment
Dry ventilation: Peristaltic pump should be stored in a dry, well-ventilated place to avoid corrosion or aging of parts caused by damp environment.
Avoid direct sunlight: Long time direct sunlight will make the pump body material aging, so it should be stored in a cool place.
Non-corrosive gases: Corrosive gases should be avoided in the storage environment, which will cause damage to the pump material.
Parameter
Applications in tissue processing
The dehydration process
Dehydration is a crucial step in tissue treatment. The main purpose of the dehydration process is to remove water from the tissue so that subsequent wax dipping and sectioning steps can proceed smoothly. The peristaltic pump ensures uniform dewatering of tissue samples by precisely controlling the delivery rate and amount of dehydrant. This precise control helps to avoid inhomogeneity in the dehydration process, thereby improving the efficiency and quality of tissue treatment.
Wax dipping process
Waxing involves dipping a dehydrated tissue sample into molten paraffin wax in order to preserve the shape and integrity of the tissue when it is sliced. Peristaltic liquid pump also play a key role in this step. It can precisely control the delivery speed and amount of paraffin, ensuring that the tissue sample can be evenly soaked in paraffin. This uniform penetration helps reduce tissue damage during sectioning and improves section clarity.
Cleaning and fixing
Cleaning and fixing are also essential steps in the tissue treatment process. The cleaning process helps to remove impurities and contaminants from the surface of the tissue, while the fixing process helps to maintain the shape and structure of the tissue. Peristaltic pumps can be used to transport cleaning and fixing fluids, and by precisely controlling the flow rate and time, you can ensure the uniformity and consistency of cleaning and fixing. This uniformity and consistency helps to improve the stability and reliability of tissue processing.
Other applications
In addition to the above applications, peristaltic pumps can also be used for other tissue treatment steps, such as pre-treatment before tissue sections, pre-treatment before tissue staining, etc. In these steps, peristaltic pumps can also ensure uniform treatment of tissue samples by precisely controlling the speed and amount of fluid delivered.
Applications in decolorization and redyeing
Decolorization process
Precise control of decolorizing fluid delivery
The peristaltic liquid pump can precisely control the conveying speed and amount of the decolorizing liquid, ensuring that the decolorizing process can be carried out evenly and consistently. During the decolorization process, the decolorization solution needs to be applied evenly to the tissue sample to remove excess stain or background color. The precise control function of the peristaltic pump allows this process to be implemented precisely, thus improving the quality and efficiency of the decolorization.
Avoid excessive decolorization
Excessive decolorization may result in changes in the structure and morphology of tissue samples, thus affecting the accuracy of staining results. The peristaltic pump can avoid the occurrence of excessive decolorization by precisely controlling the conveying time and amount of decolorization liquid. This helps maintain the integrity of the tissue sample and provides a good basis for subsequent restaining steps.
The redyeing process
Precise control of redyeing fluid delivery
In the re-dyeing process, the peristaltic pump can also accurately control the transmission speed and amount of the re-dyeing liquid. This helps to ensure that the redye is applied evenly and accurately to the tissue sample to achieve the desired staining effect.
Improve the efficiency of redyeing
The precise control function of the peristaltic pump makes the redyeing process efficient. By optimizing the delivery parameters, the peristaltic pump can quickly deliver the redyeing solution to the tissue sample, thereby reducing the redyeing time and improving the dyeing efficiency.
Reduce human error
The use of peristaltic pump can reduce the occurrence of human error. Because the peristaltic pump has a precise transmission control function, it can avoid the error and uncertainty caused by manual operation. This helps to ensure the accuracy and consistency of the staining results.
Application in gene sequencing
Sample delivery
In the genetic sequencing process, the delivery of samples (such as DNA fragments) is one of the key steps. The peristaltic pump can precisely control the delivery speed and flow rate of the sample to ensure that the sample is evenly distributed in the sequencing reaction, thereby improving the accuracy and reliability of sequencing.
Reagent addition
In the process of gene sequencing, a variety of reagents need to be added, such as sequencing primers, DNA polymerase, dNTP (deoxynucleotide triphosphate), etc. The peristaltic pump can precisely control the amount and time of addition of these reagents, ensuring that the sequencing reaction is carried out under optimal conditions.
System cleaning
After completing one sequence, the sequencing system needs to be cleaned to prevent the residue from interfering with subsequent experiments. The peristaltic pump can be used to deliver cleaning fluid to ensure the cleanliness of the sequencing system.
Disinfectant soaking time
Peristaltic liquid pump (peristaltic pump) in the chemical disinfection, the disinfectant needs to soak the time depends on the type of disinfectant selected, the concentration, and the material and contamination of the peristaltic pump components. Here are some common disinfectant soaking times for reference:
Chlorine-containing disinfectant:
If you choose to use a chlorine-containing disinfectant, a soak time of 30 minutes is usually recommended.
In this process, it is necessary to ensure that the solution temperature is not lower than 25 ° C, and add equal amounts of disinfectants according to the proportion.
Hydrogen peroxide disinfectant:
For hydrogen peroxide disinfectants, the soak time is usually 15 minutes.
At this time, the concentration of the disinfection solution should not be lower than a certain standard (such as 1000ppm), and the temperature should not be lower than 20℃.
Other chemical disinfectants:
For other types of chemical disinfectants, the soaking time may vary depending on the type and concentration of the disinfectant.
Before use, read the manual carefully and follow the operating procedures and soaking time recommendations.
It should be noted that the soaking time is not the longer the better. Too long a soaking time can lead to corrosion or damage to components, while also increasing the waste and cost of disinfectants. Therefore, when choosing the soaking time, a reasonable choice should be made according to the actual situation and the characteristics of the disinfectant.
In addition, during the soaking process, it is also necessary to ensure that the peristaltic pump parts are completely immersed in the disinfectant, and to avoid squeezing or overlapping between the parts to ensure full contact and uniform penetration of the disinfectant. At the same time, after the completion of soaking, the parts should be washed with water or appropriate solvents to remove the residual disinfectant, and in a dry and ventilated place to dry naturally or use a clean cloth to dry.
High temperature disinfection
High-temperature disinfection procedure
Preparatory work
Disconnect the peristaltic pump and ensure that the equipment is in the state of power failure.
Disassemble the hose, pump and other components of peristaltic pump for separate disinfection.
Clean the surface of the peristaltic pump and disassembled parts to remove dirt and residue.
High temperature disinfection
Place the removed parts in a container that can withstand high temperatures, such as an autoclave.
Set the appropriate temperature and time parameters according to the operating procedures of the selected high-temperature disinfection equipment. In general, the temperature of high-temperature steam disinfection should reach 121 ° C or higher, and the duration is usually 15-30 minutes to ensure the killing of microorganisms such as bacteria, viruses and spores.
Activate the high-temperature disinfection equipment to begin the disinfection process. During the disinfection process, make sure the container is well sealed to prevent steam leakage.
Cooling and drying
After disinfection is complete, turn off the high-temperature disinfection equipment and allow the container to cool naturally to room temperature.
Remove the peristaltic pump parts from the container and place in a dry and ventilated place to dry naturally or wipe dry with a clean cloth.
Precautions
Material compatibility
Before sterilizing, make sure that the material of the peristaltic pump and hose can withstand the temperature and pressure of the selected sterilizing equipment. Generally speaking, the silicone hose has good high temperature resistance and is suitable for high temperature steam disinfection.
Personal protection
When performing high-temperature disinfection, operators should wear appropriate personal protective equipment, such as gloves, masks, and goggles, to prevent skin and eye irritation or injury caused by steam.
Equipment maintenance
Regular inspection and maintenance of high-temperature disinfection equipment to ensure its normal operation and safety.
After using the high-temperature disinfection equipment, it is necessary to clean and maintain the equipment in time to extend its service life.
Verification of disinfection effect
After each high temperature disinfection, biological indicators or chemical indicators can be used to verify the disinfection effect. Biological indicators usually contain a known number of microorganisms, and disinfection is considered effective if the number of microorganisms surviving after disinfection falls below a certain level. Chemical indicators, through color changes, indicate whether the disinfection process has reached a predetermined temperature and time.
Conclusion
Peristaltic liquid pumps offer a compelling combination of precision, reliability, and versatility, making them indispensable in industries where fluid integrity and process control are paramount. While challenges such as tube wear and pulsatile flow persist, ongoing advancements in materials science, automation, and design are addressing these limitations. As industries demand safer, more efficient, and sustainable fluid handling solutions, peristaltic pumps are poised to play an increasingly prominent role in shaping the future of process engineering.
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