Hand Centrifuge
Description
Technical Parameters
Hand centrifuge is a kind of experimental equipment that separates or concentrates samples by using centrifugal force by manually shaking the handle to make the rotor in the centrifuge rotate at high speed. Its working principle is based on the action of centrifugal force. By manually shaking the handle, the internal rotor is driven to rotate at high speed. During the rotation process, different components in the sample will be subjected to different amounts of centrifugal force due to differences in density and mass, so as to achieve separation or concentration, which has a wide range of applications in biology, medicine and other fields. For example, it can be used to check for urine deposits, concentrated feces for certain parasites, and so on. However, due to its slower rotation speed, it may be difficult to cope with some high-speed separation needs.
As a kind of equipment to realize sample separation or concentration by manual operation, this equipment has certain application value in laboratory. However, due to its manual operation characteristics, special attention needs to be paid to some matters when using to ensure the safety and effectiveness of the experiment.
Usage method
Preparation before use
Check the equipment
Ensure that the connection parts of the hand centrifuge are firm and not loose.
Check that the rotor is installed correctly and tighten the setting screws.
Confirm that the centrifuge is placed smoothly, and the four rubber feet should firmly contact the plane.
Prepare sample
Prepare the samples to be separated according to the experimental requirements, and prepare them according to the specified proportions and methods.
Ensure that the sample volume is moderate and does not exceed the capacity limit of the centrifuge tube.
Install the rotor and load the sample
Install rotor
Install the rotor of the device onto the centrifuge to ensure that the rotor is securely installed.
Select the appropriate rotor type according to the experimental requirements, such as Angle rotor or tube rotor.
Loading sample
The prepared sample is evenly placed into the centrifugal tube of the rotor.
Pay attention to the balance and symmetry of the sample. If only one test tube is used, the other tube should contain an equal weight liquid as a counterweight.
Start centrifugation
Shake the handle
Turn the handle clockwise to start the centrifuge.
Adjust the centrifugal speed and time according to the experimental requirements to achieve the best separation effect.
Monitoring process
During the centrifuge process, pay attention to the running state of the centrifuge to ensure that there is no abnormal vibration or noise.
If abnormal conditions are found, the centrifugation should be stopped immediately and the cause examined.
End centrifugation and take out sample
Stop centrifugation
When the centrifugal time reaches the preset value, stop shaking the handle.
Wait for the centrifuge to stop spinning completely before proceeding to the next step.
Take out the sample
Carefully remove the rotor and remove the centrifugal tube.
Do not forcibly remove the sample when the centrifuge has not completely stopped rotating, so as to avoid sample spatter or equipment damage.
Cleaning and storage
Cleaning equipment
After each experiment, the centrifuge is cleaned and inspected.
Wipe the surface of the equipment with a clean cloth to remove dirt and residue.
For centrifugal tubes and other accessories, rinse with water and dry.
In good storage
Store the equipment in a dry, ventilated place to avoid moisture and corrosion.
If the equipment needs to be put aside for a long time, it should be checked and maintained regularly to ensure that it is in good working condition.
Precautions
Safe operation
When using the equipment, follow laboratory safety procedures and wear necessary personal protective equipment.
Do not open the cover or touch the rotating parts while the centrifuge is running.
Avoid overload
Do not place samples that are too heavy or items that exceed its carrying capacity on the device.
Periodic calibration
If the device is equipped with measuring equipment such as a tachometer, it should be calibrated regularly to ensure the accuracy of the measurement results.
Troubleshoot in time
If the equipment is found to be abnormal or faulty, it should be repaired or replaced in time to avoid further damage or accidents.
Precautions for use
Preparations before the operation
Familiar with the operation
Before use, the operator should be familiar with all the operating processes and possible accidents, and strictly follow the instructions.
Check the equipment
Check whether the connecting parts are firm to ensure that the equipment will not be loose during use and cause safety hazards.
Check whether the rotor is installed correctly and firmly to avoid accidents such as rotor falling off during the centrifugal process.
Prepare sample
Prepare the samples to be separated according to the experimental requirements, and prepare them according to the specified proportions and methods.
Ensure that the sample quantity is moderate to avoid too much or too little resulting in poor centrifugal effect or equipment damage.
Precautions during operation
Balanced loading
When loading the sample, ensure the balance and symmetry of the sample. If only one test tube is used, the other tube should be filled with an equal weight liquid as a counterweight to avoid excessive vibration during centrifugation.
01
Shake the handle correctly
Turn the handle clockwise to start the centrifuge. When shaking the handle, keep the rhythm stable to avoid damage to the equipment caused by sudden acceleration or deceleration.
Pay attention to the rotation and judge whether the handle is in normal working condition by the feel.
02
Control centrifugal time and speed
Adjust the centrifugal time and speed according to the experimental requirements to achieve the best separation effect.
Be careful not to over-centrifuge, so as not to damage the sample or affect the experimental results.
03
Avoid overload
Do not place too heavy samples or items that exceed its carrying capacity on the hand centrifuge, so as to avoid damage to the equipment or safety accidents.
04
Handling after operation
Take out the sample
At the end of centrifugation, stop shaking the handle, and when the centrifuge has completely stopped rotating, remove the rotor and carefully remove the sample in the centrifuge tube.
Do not forcibly remove the sample when the centrifuge has not completely stopped rotating, so as to avoid sample spatter or equipment damage.
Cleaning equipment
After each experiment, the centrifuge is cleaned and inspected. Wipe the surface of the equipment with a clean cloth to remove dirt and residue.
For centrifugal test tubes and other accessories, rinse with water and dry.
In good storage
When not in use, it should be stored in a dry and ventilated place to avoid moisture and corrosion.
If the equipment needs to be put aside for a long time, it should be checked and maintained regularly to ensure that it is in good working condition.
Other precautions
Avoid collisions and falls
When using or storing the device, avoid collision or falling, which may damage the device.
Periodic calibration
If the device is equipped with measuring equipment such as a tachometer, it should be calibrated regularly to ensure the accuracy of the measurement results.
Compliance with safety regulations
When using the equipment, you should follow laboratory safety procedures and wear necessary personal protective equipment, such as laboratory clothing and gloves.
Timely maintenance
If the equipment is found to be abnormal or faulty, it should be repaired or replaced in time to avoid further damage or accidents.
Care and maintenance
Daily cleaning
Centrifuge housing: After each experiment, wipe the centrifuge housing with a clean cotton cloth to remove dirt and residue and keep the equipment clean.
Centrifugal ventricle and rotor:
Clean the centrifuges and rotors regularly (e.g. after each experiment or weekly) to remove sample residue.
Use neutral detergent or special cleaning agent, avoid the use of strong acid, alkali and other corrosive substances.
After cleaning, dry with a soft cloth to prevent rust caused by residual moisture.
Rotor maintenance
Handle lightly
When installing and removing the rotor, handle gently to avoid bumps and damage.
Lubrication maintenance
Regular (such as every six months) lubrication maintenance of the rotor drive shaft, apply a little medical Vaseline or other grease to ensure that the rotor runs smoothly.
Inspection and replacement
Regularly check whether the rotor has corrosion spots, grooves, small cracks and other signs of damage, if there should be timely replacement.
Equipment inspection
Fastening bolts
Regularly check whether the fastening bolts in each part of the centrifuge are loose, and tighten them in time if they are loose.
Rubber sealing ring
Check whether the rubber sealing ring at the contact between the upper part of the centrifuge chamber and the door cover, the rotor seat and the bottom of the centrifuge chamber are deformed or aging, if any, it should be replaced in time.
Electrical system
Check the dryness of the control box, whether the cable connection is firm, the tightness of the explosion-proof part, etc., to ensure the normal operation of the electrical system.
Safe storage
Dry ventilation: When the hand centrifuge is not used, it should be stored in a dry, ventilated place to avoid moisture and corrosion.
Dust proof measures: If the device needs to be left unused for a long time, cover it with a dust proof cover to prevent dust accumulation.
Regular calibration and maintenance
Speed calibration: The speed of the centrifuge is calibrated regularly (such as annually) to ensure the accuracy of the experimental results.
Professional maintenance: If you find that the hand centrifuge is abnormal or faulty, you should contact professionals in time to repair or replace the parts to avoid equipment damage caused by disassembly.
Operation specification
Balanced loading
When loading the sample, ensure the balance and symmetry of the sample to avoid excessive vibration during the centrifugal process.
Avoid overload
Do not place samples that are too heavy or items that exceed its carrying capacity on the device.
Correct operation
Operate the manual centrifuge in strict accordance with the instructions to avoid equipment damage or safety accidents caused by illegal operations.
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