Large Glass Chromatography Columns

The large glass chromatography column is mainly composed of column tube, pressure cap, sleeve (sealing ring), screen plate (filter), joint, screw and other components. The column tube is made of stainless steel or glass, of which the glass material has high corrosion resistance and stability, and can withstand the erosion of various chemical agents, so it is widely used in organic synthesis, environmental monitoring and other fields. In addition, the surface of the glass column is smooth and the adsorption force on the sample is small, which is conducive to improving the analysis accuracy. However, glass columns are expensive to manufacture and tend to break during use, requiring careful handling.

The selection of this equipment should be centered on the separation target, and a systematic assessment should be conducted in combination with the experimental scale, packing material characteristics, equipment compatibility and safety regulations. By optimizing the inner diameter, column length, packing type and operating conditions, efficient and stable separation effects can be achieved, meeting the diverse demands from method development to industrial preparation.

As an important part of chromatographic separation technology, large glass chromatography columns play a key role in many fields. Its intuitiveness, chemical stability, and wide range of applications make it the tool of choice for laboratory and industrial scale separation and purification processes. The following is a detailed analysis of the specific application cases of large glass columns in various fields.

► Biopharmaceutical field
In the field of biopharmaceuticals, large glass columns are widely used in the process of drug separation and purification. Since biopharmaceutical products often have extremely high requirements for purity and impurity content, column selection and performance are critical.

a. Protein purification
1) Case Background: In the biopharmaceutical process, protein purification is a key step. Large glass chromatographic columns are often used for the separation and purification of proteins because of their chemical stability and high efficiency.
2) Application example: A biopharmaceutical company used large glass chromatographic column to purify recombinant protein. By selecting suitable conditions of fixed phase and mobile phase, the efficient separation of target protein was successfully achieved. The column not only has excellent separation effect, but also can effectively remove impurities and improve the purity of the product.
3) Technical features: In the process of protein purification, the large glass column is usually designed with a jacket to control the temperature and ensure the stability and repeatability of the separation process. At the same time, its efficient separation capacity and large processing capacity make the technology have significant advantages in industrial production.
b. Antibody drug isolation
1) Case background: Antibody drugs are an important branch of biopharmaceutical field, and their separation and purification process also has strict requirements on chromatographic columns.
2) Application example: A large glass chromatographic column was used to separate and purify monoclonal antibody in an antibody drug manufacturer. By optimizing the chromatographic conditions, the high efficiency of antibody separation and purification was successfully achieved. The chromatographic column not only has excellent separation effect, but also can effectively remove impurities such as antibody fragments and polymers, and improve the quality and stability of the product.
3) Technical features: In the process of antibody drug separation, large glass columns usually use stationary phases such as ion exchange or affinity chromatography to improve the separation efficiency and purity. At the same time, its large processing capacity and stable separation performance make the technology have a wide application prospect in the production of antibody drugs.
► Environmental monitoring
In the field of environmental monitoring, large glass columns are used to analyze pollutants in the air, organic pollutants in water, etc. Its efficient separation capacity and stable performance provide reliable technical support for environmental monitoring.

a. Analysis of air pollutants
1) Case background: With the acceleration of industrialization and urbanization, the types and concentrations of pollutants in the air are increasing. In order to accurately assess air quality, an accurate analysis of pollutants in the air is needed.
2) Application example: A large glass chromatographic column was used to analyze volatile organic compounds in the air in an environmental monitoring organization. By selecting suitable chromatographic conditions and detector, the accurate detection of benzene, toluene, xylene and other volatile organic compounds in the air was successfully realized.
3) Technical features: In the process of air pollutant analysis, large glass columns are usually used by gas chromatography, which has the advantages of high sensitivity and good separation effect. At the same time, its large processing capacity and stable separation performance make the technology have a wide application prospect in environmental monitoring.
b. Analysis of organic pollutants in water
1) Case background: Organic pollutants in water have a serious impact on water quality and ecological environment. In order to accurately assess the water quality, it is necessary to accurately analyze the organic pollutants in the water.
2) Application example: A water company used a large glass chromatographic column to analyze organic pollutants in water. By optimizing chromatographic conditions and selecting suitable detector, the accurate detection of organic pollutants such as phenols, chlorinated hydrocarbons and organophosphorus pesticides in water was successfully realized.
3) Technical features: In the process of organic pollutants analysis in water, large glass columns are usually used by liquid chromatography or gas chromatography-mass spectrometry, which has the advantages of good separation effect and high sensitivity. At the same time, its large processing capacity and stable separation performance make the technology play an important role in water quality monitoring and ecological protection.
► Petrochemical industry
In the field of petrochemical industry, large glass columns are used to analyze components in petroleum products and impurities in catalysts. Its efficient separation capacity and the ability to handle complex samples provide strong support for the quality control and research and development of petrochemical products.

a. Composition analysis of petroleum products
1) Case Background: Petroleum products are complex mixtures of various hydrocarbon compounds. In order to accurately evaluate the quality and performance of petroleum products, it is necessary to carry out accurate analysis of their components.
2) Application example: A large glass chromatographic column was used to analyze the components of petroleum products such as gasoline and diesel oil in a petrochemical enterprise. By selecting suitable chromatographic conditions and detectors, the accurate detection of hydrocarbons in petroleum products has been successfully achieved.
3) Technical features: In the process of petroleum product component analysis, the large glass column is usually used by gas chromatography or liquid chromatography, which has the advantages of good separation effect, high sensitivity and good reproducibility. At the same time, its large processing capacity and stable separation performance make the technology have a wide application prospect in the quality control and research and development of petrochemical products.
b. Catalyst impurity analysis
1) Case background: Catalyst is a key material in petrochemical process. Its purity and impurity content directly affect the catalytic effect and product quality. Therefore, it is necessary to accurately analyze the impurities in the catalyst.
2) Application example: A large glass chromatographic column was used to analyze the impurities in the catalyst. By optimizing chromatographic conditions and selecting suitable detector, the accurate detection of metal ions, organic matter and other impurities in catalyst was successfully realized.
3) Technical features: In the process of catalyst impurity analysis, the large glass column usually adopts the stationary phase such as ion exchange chromatography or gel chromatography to improve the separation efficiency and purity. At the same time, its large throughput and stable separation performance make this technology play an important role in catalyst production and quality control.
► Food testing field
In the field of food testing, large glass columns are used to analyze nutrients, additives, pesticide residues in food. Its efficient separation capacity and ability to handle complex samples provide a strong guarantee for food quality and safety.

a. Nutrient analysis
1) Case background: The nutritional composition of food is an important index to evaluate the nutritional value of food. In order to accurately understand the nutritional content of food, it is necessary to carry out accurate analysis.
2) Application example: A food testing institution uses a large glass chromatographic column to analyze nutrients such as vitamins and amino acids in food. By selecting suitable chromatographic conditions and detectors, the accurate detection of nutrients in food was successfully realized.
3) Technical features: In the process of nutrient composition analysis, large glass columns are usually used by liquid chromatography or gas chromatography-mass spectrometry, which has the advantages of good separation effect, high sensitivity and good reproducibility. At the same time, its large processing capacity and stable separation performance make the technology have a wide application prospect in food nutrition analysis.
b. Pesticide residue analysis
1) Case background: Pesticide residue is one of the important factors affecting food safety. In order to protect the health rights and interests of consumers, it is necessary to accurately analyze the pesticide residues in food.
2) Application example: A large glass chromatographic column was used to analyze pesticide residues in agricultural products. By optimizing chromatographic conditions and selecting suitable detector, the accurate detection of organophosphorus pesticide and pyrethroid pesticide residues in agricultural products was successfully realized.
3) Technical characteristics: In the process of pesticide residue analysis, the large glass chromatography column is usually used by liquid chromatography or gas chromatographoly-mass spectrometry, which has the advantages of good separation effect, high sensitivity and good reproducibility. At the same time, its large processing capacity and stable separation performance make the technology play an important role in the detection of pesticide residues in agricultural products.

Step:

 After separation, rinse 3 to 5 times the column volume with pure methanol or acetonitrile to remove strongly retained impurities.

 If a polar stationary phase (such as silica gel) is used, a transition flushing with isopropyl alcohol - water (50:50) is required to prevent the packing from swelling.

Frequency: Executed after each separation to avoid cross-contamination.

Applicable scenarios: When column efficiency drops (theoretical plate number decreases by more than 30%) or peak shape tailing.

Method:

 Reverse flushing: Use a strong solvent 20 times the column volume (such as dichloromethane or chloroform) to pass through the chromatographic column in reverse at a flow rate of 0.5-1 mL/min to dissolve and elute the residual impurities.

 Acid and alkali treatment (only acid and alkali resistant fillers) :

Acidic regeneration: Rinse 5 times the column volume with 0.1M hydrochloric acid solution to neutralize alkaline impurities.

Alkaline regeneration: Rinse five times the column volume with 0.1M sodium hydroxide solution to remove acidic substances.

Note: After treatment, it should be rinsed with pure water until neutral, and then balanced with an organic solvent.

Verification: After regeneration, test the resolution and peak shape with standard substances to confirm the recovery of column efficiency.

Step:

 Packing material storage: Fill the chromatographic column with 20% ethanol solution, and seal both ends with silanized glass wool to prevent microbial growth.

 Storage conditions: Store at 4-25℃ away from light and avoid drastic temperature fluctuations.

Note: Before storage, it is necessary to confirm that the packing material is compatible with ethanol. Some ion exchange resins may need to be stored with water or salt solutions.

In summary, large glass columns play an important role in many fields such as biopharmaceutical, environmental monitoring, petrochemical, food testing and so on. Its efficient separation capacity, stable performance and wide range of applications make this technology a significant advantage in laboratory and industrial scale separation and purification processes.

With the continuous progress of science and technology and the continuous development of chromatography technology, the performance and application range of large glass chromatography columns will be further improved and expanded. For example, the separation efficiency and selectivity of chromatographic columns can be improved by improving the stationary phase materials and preparation processes. By optimizing chromatographic conditions and detector technology, the sensitivity and accuracy of the analysis can be improved. Through the introduction of intelligent and automatic technology, the automatic control and data processing of chromatographic separation process can be realized.

In the future, large glass columns will be widely used and promoted in more fields, and make greater contributions to scientific research, industrial production and social progress. At the same time, it is also necessary to continuously strengthen technological innovation and personnel training to promote the sustainable development and progress of chromatography technology.

Hot Tags: large glass chromatography columns, China large glass chromatography columns manufacturers, suppliers, factory, High Pressure Stainless Steel Reactor, Glass Lab Reactor, 10l Rotovap, Teflon Lined Hydrothermal Autoclave, Rotary Evaporators, Zp9 Rotary Tablet Press