small conical flask
1) Narrow-mouth Bottle: 50ml~10000ml;
2) Big B Bottle: 50ml~3000ml;
3) Horn Mouth: 50ml~5000ml;
4) Wide-mouth Bottle: 50ml/100ml/250ml/500ml/1000ml;
5) Conical Flask With Cover: 50ml~1000ml;
6) Screw Conical Flask:
a. Black Lid (General Sets): 50ml~1000ml
b. Orange Lid (Thickening Type): 250ml~5000ml;
2. Single and Multi-mouth Round Bottom Flask:
1) Single Mouth Round Bottom Flask: 50ml~10000ml;
2) Inclined Three-mouth Flask: 100ml~10000ml;
3) Inclined Four-mouth Flask: 250ml~20000ml;
4) Straight Three-mouth Flask: 100ml~10000ml;
5) Straight Four-mouth Flask: 250ml~10000ml.
***Price List for whole above, inquire us to get
Description
Technical Parameters
The small conical flask is a versatile and efficient tool that plays a critical role in modern science and laboratory practices. Its cone-shaped design, narrow neck, and wide base make it ideal for a variety of reactions and culturing processes. Additionally, its durability, ease of cleaning, and visual monitoring capabilities enhance its utility in scientific research.
As scientific research continues to evolve, the mini conical flask will undoubtedly play an increasingly important role in advancing our understanding of the natural world. Its combination of functionality, practicality, and ease of use makes it a indispensable tool in the hands of researchers and scientists. The future of the mini conical flask is bright, and its contributions to scientific research will undoubtedly continue to grow and expand in the coming years.
Design and Features
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A small conical flask, often referred to as an Erlenmeyer flask due to its inventor, is characterized by its narrow neck and broad, conical base. This design serves several purposes, enhancing both the functionality and efficiency of the flask. The narrow neck prevents the loss of volatile compounds and allows for easy pouring and handling, while the broad base provides stability and sufficient surface area for heating and mixing. The material composition of mini conical flasks varies, depending on the specific application and the chemical or biological agents involved. Common materials include glass, plastic, and even stainless steel. Glass flasks are preferred for their chemical inertness, clarity, and durability, making them ideal for reactions involving high temperatures or aggressive chemicals. Plastic flasks, on the other hand, offer a lightweight and disposable alternative, which is particularly useful in applications where sterilization or cross-contamination is a concern. Stainless steel flasks are often used in industrial settings, where durability and corrosion resistance are critical. The capacity of mini conical flasks ranges from a few milliliters to several liters, catering to the diverse needs of researchers. Smaller flasks are convenient for preliminary experiments, where small sample sizes are sufficient, while larger flasks are essential for scaling up reactions or cultivations. |
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Specifications
Uses in Chemical and Biological Research
The versatility of the small conical flask is evident in its wide range of applications in both chemical and biological research. In chemistry, mini conical flasks are commonly used for reactions that require stirring, heating, or the addition of reactants in a controlled manner. The conical shape facilitates efficient mixing, while the narrow neck prevents splashing and the escape of harmful vapors. Furthermore, the flask's ability to withstand high temperatures makes it suitable for reactions that involve heat, such as distillation or crystallization.
In biological research, mini conical flasks are indispensable for cultivating microorganisms, such as bacteria, fungi, and yeast. The broad base provides ample surface area for aerobic growth, while the narrow neck prevents contamination from external sources. The flasks can be sterilized and sealed with cotton plugs or foil to maintain sterile conditions. Moreover, the clear material of glass flasks allows researchers to monitor the growth and morphology of microorganisms without disrupting the culture.
Beyond cultivations, mini conical flasks are also used in biochemical experiments, such as enzyme assays and protein purification. The flasks can be adapted for use in centrifuges, enabling the separation of cellular components or the purification of proteins. Additionally, they serve as containers for storing buffers, reagents, and other solutions needed for various experimental procedures.
Application of small conical bottles in experimental steps
► Production of standard curve:
Take a certain amount of standard amino acid solution in a test tube, and make it up to a certain volume with distilled water.
Add a certain amount of acetic acid buffer and ninhydrin color developing solution, fully mixed, heated in a water bath at a specific temperature, and then cooled.
After ethanol was added and diluted, the absorbance was measured by spectrophotometer and the standard curve was drawn.
In this process, although the small conical bottle is not directly used for the production of standard curves, it can usually be used as a storage and mixing container for reagents to ensure the accuracy and consistency of the experiment.
► Amino acid sample determination:
Take a certain amount of sample liquid (such as egg white diluent) in a small conical bottle.
Add acetic acid buffer and ninhydrin coloring solution, mix well, and heat and cool in water bath.
After ethanol was added and diluted, the solution was transferred to a colorimetric dish and the absorbance was measured by spectrophotometer.
Calculate the amino acid or protein content of the sample based on the standard curve and measured absorbance.
The Role of Mini Conical Flasks in Modern Scientific Research
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The small conical flask has become an indispensable tool in modern scientific research, playing a crucial role in advancing our understanding of the natural world and solving complex problems. Here are some of the ways in which mini conical flasks contribute to scientific progress: Facilitating Precise Measurements and ControlThe accuracy and reproducibility of experimental results are crucial for advancing scientific knowledge. Mini conical flasks, with their precise graduations and durable materials, enable researchers to measure and control experimental conditions with a high degree of precision. This ensures that the results of experiments are reliable and can be replicated by other researchers, fostering collaboration and the sharing of knowledge. Supporting Advanced Analytical TechniquesThe versatility of mini conical flasks makes them suitable for a wide range of analytical techniques, such as chromatography, spectroscopy, and electrophoresis. These techniques are used to separate, identify, and quantify the components of complex samples, providing valuable insights into the composition and structure of biological and chemical systems. Enabling High-Throughput ScreeningIn drug discovery and materials science, high-throughput screening involves testing a large number of compounds or materials to identify those with desired properties. Mini conical flasks are ideal for this purpose, as they allow researchers to conduct multiple experiments simultaneously, reducing the time and cost associated with drug development and materials research. |
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Case Studies: Illustrating the Importance of Mini Conical Flasks
Case Study 1: Synthesis of Organic Compounds
In the synthesis of organic compounds, researchers often rely on mini conical flasks to carry out reactions under controlled conditions. For example, in the synthesis of a new drug molecule, reactants are mixed in a flask, heated to a specific temperature, and stirred for a defined period. The conical shape of the flask ensures efficient mixing and uniform heating, leading to higher yields and purer products.
Case Study 2: Cultivation of Microorganisms
In the field of microbiology, mini conical flasks are essential for cultivating microorganisms under sterile conditions. For instance, in the study of antibiotic resistance, bacteria are grown in flasks containing various concentrations of antibiotics. The flasks are incubated at a controlled temperature, and the growth of bacteria is monitored over time. The conical shape of the flasks provides ample surface area for aerobic growth, while the narrow neck prevents contamination from external sources.
Case Study 3: Enzyme Activity Assays
In biochemistry, small conical flasks are used to measure the activity of enzymes. For example, in an assay to determine the activity of a specific enzyme, a substrate is mixed with the enzyme in a flask, and the rate of product formation is measured. The conical shape of the flask ensures efficient mixing of the substrate and enzyme, leading to more accurate and reproducible results.
Innovations in Mini Conical Flask Design
Recent advancements in glassware technology have led to the development of innovative mini conical flask designs. For example, some flasks are now equipped with integrated stirrers and heating elements, enabling researchers to automate mixing and heating processes. Additionally, some flasks are made from specialized materials, such as zirconia or quartz, which offer enhanced chemical resistance and thermal stability.
These innovations have expanded the utility of the mini conical flask, making it suitable for a wider range of experimental setups. For example, flasks equipped with integrated stirrers and heating elements are ideal for reactions that require precise temperature control and continuous mixing. Similarly, flasks made from specialized materials are suitable for reactions involving aggressive chemicals or high temperatures.
Esterification reaction
Principle of esterification reaction
Esterification is a reversible reaction with the formula R-COOh + R'-OH → R-Coor '+ H2O, where R-COOh represents organic acid, R'-OH represents alcohol, r-Coor' represents ester, and H2O is water. In order to improve the efficiency and yield of the esterification reaction, it is usually necessary to add a catalyst, such as concentrated sulfuric acid, which can not only provide the acidic environment required for the reaction, but also absorb the generated water and promote the reaction in the direction of ester formation. At the same time, appropriately increasing the reaction temperature can accelerate the reaction rate, but too high temperature may lead to side reactions or product decomposition, so temperature control is needed.
The application of small conical bottle in esterification reaction
As a container for esterification, the small conical bottle has many advantages. Its conical structure enables the reactants to be fully mixed, and is conducive to observing experimental phenomena such as color change and bubble formation during the reaction. At the same time, the material of the small conical bottle is usually high-quality glass, which can resist high temperature and corrosion, and is suitable for heating and stirring operations in the esterification reaction.
In the esterification reaction, the use of small conical bottles is as follows:
Prepare the reactants: Add the appropriate amount of acid and alcohol and catalyst to a small conical bottle. For example, in the experiment of preparing ethyl acetate, ethanol, glacial acetic acid and concentrated sulfuric acid can be added to the conical bottle in a certain proportion.
Heating and stirring: Place a small conical bottle on a heating device, such as an electric jacket or a water bath, and heat it. At the same time, stir using a magnetic stirrer or a manual stirring rod to ensure that the reactants are well mixed. The heating temperature should be controlled within an appropriate range to avoid the occurrence of side reactions.
Observe the reaction phenomenon: During the reaction process, closely observe the experimental phenomena such as color change and bubble generation in the small conical bottle. These phenomena can provide information about the degree and rate of reaction.
Separation and purification: After the reaction, the target ester product is obtained through the appropriate separation and purification steps. For example, the resulting ethyl acetate can be separated from the reaction mixture by steam distillation and further purified.
Precautions
Safe operation
When carrying out esterification reaction, it is necessary to pay attention to the safe use of chemicals. Catalysts such as concentrated sulfuric acid are highly corrosive. Wear protective gloves and goggles to avoid direct contact with skin and eyes. At the same time, the experimental waste liquid needs to be properly disposed of and cannot be discharged directly into the environment.
Temperature control
Esterification is an exothermic reaction, so the reaction temperature needs to be precisely controlled. Too high temperature may lead to the occurrence of side reactions or product decomposition, while too low temperature may reduce the reaction rate. Generally, the temperature of esterification reaction is controlled between 110 and 120℃.
Stirring and mixing
In esterification, adequate stirring and mixing are essential for the reaction to proceed. Stirring ensures adequate contact of the reactants and accelerates the reaction rate. Therefore, it is necessary to maintain an appropriate mixing speed and time during the experiment.
Reactant ratio
The molar ratio of reactants has an important effect on the reaction yield. In general, the use of excess alcohols can increase the yield of esters, because the excess alcohol can push the reaction equilibrium in the direction of ester formation.
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