Beaker Conical Flask
1) Narrow-mouth Bottle: 50ml~10000ml;
2) Big B Bottle: 50ml~3000ml;
3) Horn Mouth: 50ml~5000ml;
4) Wide-mouth Bottle: 50ml/100ml/250ml/500ml/1000ml;
5) Conical Flask With Cover: 50ml~1000ml;
6) Screw Conical Flask:
a. Black Lid (General Sets): 50ml~1000ml
b. Orange Lid (Thickening Type): 250ml~5000ml;
2. Single and Multi-mouth Round Bottom Flask:
1) Single Mouth Round Bottom Flask: 50ml~10000ml;
2) Inclined Three-mouth Flask: 100ml~10000ml;
3) Inclined Four-mouth Flask: 250ml~20000ml;
4) Straight Three-mouth Flask: 100ml~10000ml;
5) Straight Four-mouth Flask: 250ml~10000ml.
***Price List for whole above, inquire us to get
Description
Technical Parameters
A beaker conical flask, alternatively known as an Erlenmeyer flask (after its inventor, Erlenmeyer), is a type of laboratory flask characterized by its narrow neck and wide, conical base. This design ensures that the flask can be securely placed on a laboratory stand without tipping over and also facilitates efficient mixing of its contents.
Conical flasks are made from materials such as glass or plastic and come in various sizes and capacities. They are widely used in chemistry and biochemistry laboratories for a variety of purposes, including holding and mixing reactants, performing chemical reactions, and collecting distillation products.
A beaker, also commonly known as a large-mouthed cup, is a common piece of laboratory equipment primarily used for mixing, heating, and storing substances. It typically features a wide opening that facilitates easy pouring and stirring of contents. Made from materials such as glass or plastic, beakers are available in various sizes and capacities, suitable for different experimental needs.
Due to its wide mouth and sturdy design, a beaker is ideal for a wide range of applications in chemistry, biology, and other scientific fields. For instance, it can be used to measure and mix chemicals, heat solutions, or even as a container for holding samples during experiments.
Specifications
Applications in Biology
In DNA extraction experiments, a triangular beaker (conical flask) is commonly used to mix, stir, and heat small amounts of liquid samples. Below is a step-by-step procedure for an experiment using a triangular beaker for DNA extraction, using plant material as an example:
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Purpose of the experiment: Experimental Material: |
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Experimental Steps:
1. Sample preparation:
Grind approximately 10 g of fresh or frozen plant leaf samples into a fine powder using a mortar and pestle in liquid nitrogen.
2. Sample transfer:
Transfer the ground fine powder to an ice pre-cooled 500 mL triangular beaker containing approximately 120 mL of NIB buffer and mix quickly.
3. gentle mixing:
The contents of the triangular beaker were gently stirred on ice for 10 minutes at 100 x rpm.
4. Filtration:
The mixture was filtered through two layers of gauze and two layers of Miracloth and the remaining nuclear suspension was collected using a funnel.
5. collection of filtrate:
The filtrate was dispensed into two 50-mL triangular beakers and centrifuged at 2400 × g for 12 min at 4°C; the supernatant was discarded.
6. washing:
Using a small brush, resuspend the precipitate in each triangular beaker with 20 mL of ice-cold NIB, combine into one beaker, and centrifuge again.
7. Repeat washing:
Repeat steps 5 and 6 until the green color disappears and the suspension becomes clear.
8. DNA precipitation:
Discard the supernatant and add 0.5-2 mL of ice-cold NIB buffer to each triangular beaker and gently suspend the precipitate.
9. Addition of CTAB buffer:
Pour 20 ml of 2 × CTAB (65°C) buffer, mix immediately, and incubate at 65°C for 10 min, then cool to room temperature.
10. chloroform extraction:
Mix with an equal volume of chloroform by gently shaking or inverting and centrifuge at room temperature.
Transfer supernatant:
Transfer 20 ml of supernatant to a new tube, add 2 ml of 10% CTAB buffer, mix gently and incubate at 65°C.
Extract again with chloroform:
Extract again with chloroform and centrifuge at room temperature.
DNA precipitation:
Transfer 15 ml of supernatant to a new triangular beaker, add an equal volume of 1× CTAB precipitation buffer, mix gently by inversion to precipitate genomic DNA.
DNA resuspension:
After centrifugation, the supernatant was discarded and the DNA precipitates were suspended with 600 µl of TE high-salt solution.
DNA washing:
Transfer the suspension to a 2-mL tube, add 1.2 mL of ethanol, mix gently until the DNA precipitates, and leave at room temperature for 5 minutes.
DNA purification:
Perform further purification steps such as ethanol precipitation and resuspension as needed.
Precautions:
Always use freshly made buffer for megabase-sized DNA isolation.
Avoid excessive physical shearing when processing samples to maintain DNA integrity.
Maintain low temperature conditions during the experiment to minimize DNA degradation.
With this experiment, high quality, high molecular weight genomic DNA suitable for single-molecule sequencing can be extracted from plant samples.This method is simple, cost-effective, rapid, and does not require special equipment, and can be completed in a single day, which is a significant reduction in time compared to the traditional multi-day to one-week timeframe.
They are used in chemistry, biology, and microbiology laboratories for heating, mixing, and transferring chemicals or reagents. Specific uses include titration experiments, boiling liquids, bacterial culture preparation, and DNA extraction.
Handling of Emergencies
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When using beaker conical flask to do experiments, in the event of an emergency, the experimenteer should remain calm and take quick and accurate measures to ensure personal safety, prevent the expansion of the accident, and protect the experimental equipment and reagents as much as possible. Here are some specific responses:
Fire emergency handling
Cut off the fire immediately:
If there is a fire in or near the beaker conical flask, the fire source should be cut off immediately, such as closing the gas valve or unplugging the power plug.
Use fire extinguisher:
Choose the appropriate fire extinguisher according to the type of fire. For flammable liquid fires, such as alcohol, ether, etc., dry powder fire extinguishers or foam fire extinguishers can be used; For electrical equipment fires, the power should be cut off first, and then use a carbon dioxide fire extinguisher.
Evacuation and alarm:
If the fire cannot be controlled, the laboratory personnel should be evacuated immediately and the fire alarm phone should be called.
Pay attention to personal protection:
When fighting a fire, the experimental personnel should wear protective equipment, such as fireproof clothing, breathing apparatus, etc., to avoid injury.
Handling of explosion emergencies
Quick evacuation
If there is an explosion, the experimenter should immediately evacuate the scene to avoid secondary injury.
Cut off the power and air source
In the case of ensuring safety, quickly cut off the power and air supply to the laboratory to prevent further deterioration of the situation.
First Aid and Alarm
Give initial first aid to the injured, such as hemostasis, bandaging, etc., and make emergency calls. At the same time, report the incident to the laboratory manager and safety department.
Protect the site
Protect the accident site for subsequent investigation and analysis while ensuring safety.
Chemical reagent leakage treatment
Immediate quarantine
If the chemical reagent in the conical flask leaks, the leakage area should be immediately isolated to prevent the spread of the reagent.
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Personal Protection
Experimental personnel should wear appropriate protective equipment, such as protective clothing, gloves, goggles, etc., to avoid direct contact with leaked reagents.
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Collection and neutralization
Use appropriate collection tools (such as sand, oil absorbent cotton, etc.) to collect the leaking reagent and neutralize it with a neutralizer according to the properties of the reagent.
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Cleaning and ventilation
Clean the leaking area thoroughly and use ventilation equipment to remove harmful gases.
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General safety precautions
Familiar with laboratory safety procedures
Laboratory personnel should be familiar with laboratory safety procedures and emergency response measures so that they can respond quickly in the event of an emergency.
Regular inspection of equipment
Check laboratory equipment regularly to ensure that it is in good condition to avoid accidents caused by equipment failure.
Keep the lab clean
Keep the laboratory clean and orderly to avoid debris interfering with the experiment or causing safety risks.
Strengthen safety training
Conduct regular safety training for experimental personnel to improve their safety awareness and emergency handling ability.
Record precautions
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When conducting chemical experiments with Beaker conical bottles, recording is the key to ensuring the accuracy, traceability and scientific nature of the experimental data. A detailed, accurate and well-organized experimental record can not only provide valuable experimental data for the experimenters, but also provide a solid foundation for subsequent experimental analysis, paper writing and scientific communication. Here are some detailed notes to note when experimenting with Beaker conical bottles:
The basic principles of recording
Records should be based on actual observed experimental phenomena and data, avoiding subjective assumptions or biases. All records should be described in as objective and specific language as possible.
Recorded data should be as accurate as possible, including values, units, measurement conditions, etc. For approximations or estimates, the uncertainty should be clearly marked.
Records should cover the whole process of the experiment, from the preparation stage to the end of the experiment, including the dosage of all reagents, experimental procedures, observed phenomena, data measurement results, etc.
Records should be well organized and easy to understand. Use clear headings, sections, and lists to organize information for subsequent review and analysis.
Records should contain enough information so that other experimenters can replicate the experiment. This includes the date of the experiment, the name of the experimenter, the conditions of the experiment, the model of the instrument, etc.
Preparation of records before experiment
Experimental design: Before the experiment begins, the purpose, hypothesis, expected results, and experimental design should be recorded in detail. This helps clarify the direction and goal of the experiment.
Reagents and instruments: Record the name, purity, manufacturer, lot number of all reagents used, as well as the model, specification and calibration status of the instrument. This information is essential for the traceability of experimental results.
Safety plan: Record the possible safety risks and countermeasures in the experiment to ensure the safety of the experiment process.
Records during the experiment
Reagent addition
Record the order, amount, method and time of each reagent addition. For reagents that require accurate weighing, the exact mass should be recorded.
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Experimental procedure
Record the experimental operation of each step in detail, including heating, stirring, filtration, centrifugation, etc. For critical steps, record the specific details and precautions.
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Observed phenomena
Record all phenomena observed during the experiment, such as color changes, bubble formation, precipitation formation, temperature changes, etc. These phenomena can often reflect the process of chemical reactions and the generation of products.
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Data measurement
Record all measured data, including temperature, volume, mass, concentration, etc. For data that requires multiple measurements, the results and average values of each measurement should be recorded.
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Abnormal handling
If there is an abnormal situation during the experiment, such as reagent spatter, instrument failure, etc., the experiment should be stopped immediately and the abnormal handling process and results should be recorded.
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Record and analysis after experiment
Data collation
After the experiment, all the recorded data were collated and analyzed. Use charts, tables, and other forms to visually present the data for subsequent analysis and discussion.
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Results discussion
According to the experimental data and observed phenomena, discuss whether the experimental results meet the expectations, and analyze the possible causes and influencing factors. The results that do not meet the expectations should be deeply analyzed and discussed.
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Conclusions and Suggestions
Based on the experimental results and analysis, the experimental conclusions are drawn, and suggestions are put forward to improve the experimental method, optimize the experimental conditions or conduct further research.
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Record keeping
Keep lab records in a safe and secure place for subsequent review and sharing. Consider using electronic documents or cloud storage to keep records to improve their readability and accessibility.
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Special notes in the record
Timestamp
Adding a timestamp to the record to record the time of each key step or observation point helps to analyze the time dependence during the experiment.
Signature and Date
Each page of records should contain the signature and date of the experimenter to ensure the authenticity and traceability of the record.
Confidentiality
For experimental records involving sensitive information or patents, appropriate confidentiality measures should be taken to avoid information disclosure.
Verification of electronic records
If electronic equipment is used for recording, the accuracy and reliability of the electronic equipment should be checked regularly to ensure the accuracy of the recorded data.
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