Türkçe
Português
Việt Nam
日本語
русский 
Español
한국어
Deutsch
Français
عربي 
Italiano
Guard Column In Chromatography
2.Chromatographic Column (Rotation Type)
3.Chromatographic Column (Manual)
***Price List for whole above, inquire us to get
Description
Technical Parameters
The chromatographic protection column is an important part of the chromatographic analysis system, which is located between the injector and the analysis column, and its main function is to filter and adsorb impurities in the sample to prevent these impurities from entering and contaminating the analysis column, thereby extending the service life of the analysis column and improving the accuracy of the analysis results. Its main role is to protect the analytical column by intercepting impurities such as strong polarity, strong acid and strong alkaline substances in the sample to prevent them from contaminating and damaging the fillers in the column. This can not only extend the service life of the analysis column, but also improve the accuracy and stability of the analysis results. The device typically consists of a column sleeve and a column core, which is used to secure and protect the column core, while the column core contains fillers that are similar to those used in the analytical column to intercept impurities in the sample.
Guard column in chromatography plays an important role in chromatographic analysis system. Correct selection, use and maintenance of protective columns can not only prolong the service life of analytical columns, but also improve the accuracy and stability of analytical results.
Parameter
Methods for managing chromatographic protective columns
The management of guard column in chromatography is an important step to ensure the accuracy of chromatographic analysis and the stable operation of instruments. The following is a detailed set of methods for managing protective columns designed to help laboratory personnel effectively maintain and manage protective columns, extend their service life, and improve the efficiency and accuracy of chromatographic analysis.
Establish the use record of the protective column
Record basic information
Each time the protective column is used, the basic information such as the type, batch number, installation date, and number of uses of the protective column should be recorded in detail. This information helps track the history of the column and identify potential problems in a timely manner.
Sample and mobile phase information
Record key information such as the type and concentration of the sample used for each analysis, as well as the composition and pH of the mobile phase. This information is essential for the accuracy of the analysis results and the evaluation of the performance of the protective column.
Performance monitoring
Regularly record the column pressure, separation, peak shape and other performance indicators of the protective column. These indicators can reflect the working status and performance changes of the protective column, and help to find and solve problems in time.
Develop regular maintenance plans
Cleaning and regeneration
According to the use of the protective column and the manufacturer's recommendations, develop a regular cleaning and regeneration plan. Cleaning removes residual samples and impurities, while regeneration helps restore the performance of the protective column. When cleaning and regeneration, appropriate solvents and cleaning methods should be used to avoid damage to the protective column.
Replacement standard
Set the standard for the replacement of the protective column, such as the column pressure rise beyond a certain range, the separation degree decreases significantly or the filler falls off. Once these standards are met, the new protective column should be replaced immediately to ensure the accuracy of the analysis and the stable operation of the instrument.
Training and guidance
Operator training: Conduct training for laboratory operators on the use and maintenance of protective columns to ensure that they understand the working principle, use methods and precautions of protective columns. This helps improve the skill level of the operator and reduces damage to the protective column due to improper operation.
Provide operation manual: Provide the operator with a detailed column operation manual, including installation, use, cleaning, regeneration and replacement steps. The manual should be clear and easy to read and operate.
Inventory management
Spare parts: Maintain a certain number of spare parts for protective columns to meet emergencies or emergency requirements. Spare parts should be stored in a dry and cool place, away from direct sunlight and high temperature.
Regular inventory: Regular inventory of the protective column to ensure that the number and type of spare parts meet the needs. At the same time, check the status of spare parts, and replace expired or damaged protective columns in time.
Quality control and evaluation
Performance evaluation: Regular performance evaluation of the protective column, including column pressure, separation, peak shape and other indicators. The evaluation results should be compared with the performance parameters provided by the manufacturer to ensure that the performance of the protective column meets the requirements.
Quality control: Establish a strict quality control system to control the use, cleaning, regeneration and replacement of the protective column. Through quality control, the performance of the protective column is stable and reliable, and the accuracy and repeatability of chromatographic analysis are improved.
Matching of connection methods
Connection mode type
The connection modes between the guard column in chromatography and the analysis column mainly include manual connection, the use of connectors and the use of connecting tubes.
Manual connection:
Features: Simple and direct, the connection port of the protection column and the analysis column is aligned and closely connected by rotating.
Compatibility requirements: It is necessary to ensure that the size and shape of the connection are matched and that they fit tightly when rotated to prevent sample leakage.
Use connectors:
Features: Connectors are usually made of metal or plastic, and the protective column is connected to the analytical column by means of threads or fasteners.
Matching requirements: The connector needs to match the size and shape of the connection port of the analysis column and the protection column, and the connector itself should have good sealing and stability.
Use connecting tubes:
Features: The connecting tube is usually made of stainless steel or polymer material, and connects the protective column with the analysis column by means of thread or snap.
Matching requirements: the length, inner diameter and outer diameter of the connection pipe need to match the connection port of the analysis column and the protection column, and the connection pipe itself should have good sealing and corrosion resistance.
The importance of connection mode matching
Prevent sample leakage
Mismatched connection methods may lead to sample leakage at the connection, which affects the separation effect and the accuracy of the experimental results.
Maintain system stability
Mismatched connection modes may lead to fluctuations or instability in the operation of the chromatographic system, affecting the separation effect and the service life of the instrument.
Ensure the separation effect
AThe good matching of the connection mode can ensure that the connection between the protection column and the analysis column is tight and stable, so as to maintain the stability and consistency of the separation effect.
The selection of connection mode matching suggestions
Understand the connection mode of the analysis column
Before selecting the protection column, you need to understand the connection type and size requirements of the analysis column to ensure that the protection column is selected to match.
Consult the manufacturer's recommendations
The manufacturer will usually provide recommendations and information on how to connect the protective column to the analytical column, which can be consulted to choose the appropriate connection.
Test verification
Before formal use, tests can be carried out to verify the matching of the connection mode between the protective column and the analytical column to ensure its performance and stability in practical applications.
Precautions
Avoid using incompatible connections: Do not attempt to force incompatible connections together to avoid damaging the column or causing inaccurate experimental results.
Regular inspection of the connection method: During use, the tightness and stability of the connection method should be regularly checked to ensure that there is no leakage or loosening.
Follow the operating procedures: When connecting and disassembling the protective column, follow the operating procedures to avoid damage or failure of the connection mode caused by improper operation.
In summary, the matching of the connection mode of the protective column has an important influence on the stability and separation effect of the chromatographic system. When selecting and using the protective column, it is necessary to pay attention to the matching of its connection mode, and follow the operating procedures and manufacturer's recommendations to ensure the stability and reliability of the connection mode.
How to maintain
Periodic flushing
Purpose of flushing:
Remove accumulated impurities and residual mobile phase components from the protective column.
Maintain the separation effect and column efficiency of the protective column.
Rinse solvent selection:
Select the appropriate rinsing solvent according to the type of sample analyzed and the nature of impurities.
If the sample is mostly polar compounds, it can be washed first with a more polar solvent such as methanol or acetonitrile.
If non-polar impurities are present, then a non-polar solvent such as n-hexane can be used for subsequent flushing.
Flushing conditions:
The washing flow rate should be moderate to avoid damage to the internal structure of the protective column.
The flushing time can be adjusted according to the use of the protective column and the degree of accumulation of impurities, usually 15 to 30 minutes.
Replacement and regeneration
Replacement time:
When the column pressure of the protective column increases significantly, the separation effect decreases significantly or the baseline drift is serious, the replacement of the protective column should be considered.
Regeneration method:
For some types of protective columns, such as those based on silica gel, their performance can be restored by regenerative treatment.
Regeneration steps include flushing the protective column with a mixture of organic solvents and water, as well as cleaning with a specific solution, such as dilute sulfuric acid.
The choice of regeneration method should be determined according to the material of the protective column and the nature of the pollutant.
Storage and maintenance
Storage conditions
The protective column should be stored in a dry, cool and well-ventilated place, away from direct sunlight and high temperature and humidity environment.
High temperature may lead to aging and deformation of the packing inside the protective column, and high humidity environment is easy to rust metal parts and aging seals.
Maintenance measures
When the protective column is not used for a long time, it should be stored in an appropriate solvent, such as methanol, acetonitrile, etc., to maintain its stability and performance.
Before storage, the protective column should be thoroughly washed and dried to avoid solvent residue and bacterial growth.
Precautions
Avoid incompatible solvents:
When using the protective column, the use of incompatible solvents or mobile phases should be avoided to avoid damage to the protective column.
Regular inspection:
Check the connection status and tightness of the protective column regularly to ensure that it is in good working condition.
Record usage:
Record the use of the column, including the type of sample used, the number of analyses, the time of cleaning and maintenance, and the solvent used.
Through these records, we can better judge the performance trend of the protective column, predict the possible problems in advance and take timely measures.
In summary, the maintenance of guard column in chromatography includes regular flushing, replacement and regeneration, storage and maintenance, and precautions. Only by following the correct maintenance methods and techniques can the long-term stable operation and separation effect of the protective column be ensured.
Hot Tags: guard column in chromatography, China guard column in chromatography manufacturers, suppliers, factory, Crystallization Reactor, Laboratory Rotary Evaporator, SS Reactor Manufacturer, Single Layer Glass Reactor, Jacketed Lab Reactor, Rotating Evaporator
Send Inquiry
